Why Chemically Defined Cell Freezing Media Preserve CAR-T Viability, Recovery, and CAR Expression After Thaw

Cryopreservation is a decisive step in CAR-T and other cell therapy workflows. Between manufacturing, release testing, shipping, and clinical administration, engineered cells are frozen and thawed at least once, and every freeze-thaw cycle is an opportunity to lose viability, cell number, and, critically, the engineered function that makes the product therapeutic. For CAR-T cells, that […]
High-Density CAR-T Cryopreservation: How to Maintain Viability and Potency at 2.5 × 10^7 Cells/mL

As CAR-T and other cell therapies scale from early studies toward routine manufacturing, how cells are frozen becomes as important as how they are made. One decision has outsized impact on logistics and cost: the cell density at which the product is cryopreserved. Freezing at high concentration, for example 2.5 x 10^7 cells/mL rather than […]