Magnetic bead separationis one of the most widely used methods for isolating immune cells in immunology and immuno-oncology research. But not all magnetic beads are equal. Beyond how pure the isolated population is, a factor that often decides whether an experiment succeeds is what the beads do to the cells: whether they preserve viability, leave […]
CD8+ cytotoxic T cellsare central players in immuno-oncology. They recognize and kill tumor cells, drive responses to checkpoint inhibitors, and are the engineered effector cells behind many CAR-T and TCR-T therapies. Studying or manufacturing with these cells starts with one practical step: isolating them at high purity from a mixed population such as peripheral blood […]
Cryopreservation is a decisive step in CAR-T and other cell therapy workflows. Between manufacturing, release testing, shipping, and clinical administration, engineered cells are frozen and thawed at least once, and every freeze-thaw cycle is an opportunity to lose viability, cell number, and, critically, the engineered function that makes the product therapeutic. For CAR-T cells, that […]
As CAR-T and other cell therapies scale from early studies toward routine manufacturing, how cells are frozen becomes as important as how they are made. One decision has outsized impact on logistics and cost: the cell density at which the product is cryopreserved. Freezing at high concentration, for example 2.5 x 10^7 cells/mL rather than […]
Peripheral blood mononuclear cells (PBMCs)sit at the heart of immunology, vaccine, and drug-development research, powering immunophenotyping, functional assays, cytokine release testing, and potency studies. One of the most common questions researchers face when designing these experiments is deceptively simple: should you use fresh PBMCs or cryopreserved PBMCs? Fresh cells have long been viewed as the […]
Peripheral blood mononuclear cells (PBMCs)are one of the most widely used primary cell types in immunology, vaccine research, and drug development. They contain the lymphocytes and monocytes that drive adaptive and innate immune responses, making them an accessible, physiologically relevant window into immune function. Yet a question that shapes the entire success of a translational […]
Cryopreserved hepatocytes are a cornerstone of in vitro drug metabolism and pharmacokinetics (DMPK) research. They retain a near-complete set of Phase I and Phase II drug-metabolizing enzymes, transporters, and cofactors, making them the most physiologically relevant model for predicting hepatic clearance, profiling metabolites, and assessing drug-drug interaction potential. But not all cryopreserved hepatocytes perform equally. […]
In drug metabolism and pharmacokinetics (DMPK) research, suspension hepatocytes are widely regarded as the gold-standard in vitro model for studying hepatic clearance, metabolite profiling, and drug-drug interaction potential. They retain the full complement of Phase I and Phase II drug-metabolizing enzymes and cofactors, making them more physiologically relevant than isolated subcellular fractions. For preclinical and […]
Introduction Isolating highly purified immune cells is a foundational step in immunology and immuno-oncology research. Whether you are studying T cell function, building a co-culture model, or preparing cells for single-cell sequencing, the quality of your downstream data depends on how cleanly you can separate your target cells from a complex sample such as peripheral […]
In immunology and immuno-oncology research,how you isolate cells can be just as important as the cells themselves. When a downstream assay depends on cells that are fully functional and free of surface-bound reagents, the isolation method must avoid labeling the target population. This is exactly where negative selection kits outperform. Negative selection is a magneticcell […]
